CyFlow CD86 FITC
| 品番 | CQ603647 | ||
|---|---|---|---|
| 抗体名 | Anti-Ms CD86 FITC,GL-1 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Mouse | レーザー | Blue |
| 抗原 | CD86, B70, B7-2 | 最大蛍光波長 | 518 nm |
| クローン | GL-1 | 最大励起波長 | 490 to 495 nm |
| ホスト | Rat | 標識/Format | FITC |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms CD86 FITC,GL-1
特異性
The rat monoclonal antibody GL-1 recognizes CD86 antigen, a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
抗原情報
CD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 2 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Benschop RJ, Melamed D, Nemazee D, Cambier JC: Distinct signal thresholds for the unique antigen receptor‑linked gene expression programs in mature and immature B cells. J·Exp·Med. 1999·Sep·20; 190(6):749‑56. <·PMID:·10499913·>
• Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3‑ligand‑supplemented bone marrow cultures. Blood. 2000·Nov·1; 96(9):3029‑39. <·PMID:·11049981·>
• Chung JB, Wells AD, Adler S, Jacob A, Turka LA, Monroe JG: Incomplete activation of CD4 T cells by antigen‑presenting transitional immature B cells: implications for peripheral B and T cell responsiveness: implications for peripheral B and T cell responsiveness. J·Immunol. 2003·Aug·15; 171(4):1758‑67. <·PMID:·12902475·>
• Edgtton KL, Kausman JY, Li M, O'Sullivan K, Lo C, Hutchinson P, Yagita H, Holdsworth SR, Kitching AR: Intrarenal antigens activate CD4+ cells via co‑stimulatory signals from dendritic cells. J·Am·Soc·Nephrol. 2008·Mar; 19(3):515‑26. <·PMID:·18184859·>
• Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ: Identification of an alternative CTLA‑4 ligand costimulatory for T cell activation. Science. 1993·Nov·5; 262(5135):905‑7. <·PMID:·7694361·>
• Nolan A, Kobayashi H, Naveed B, Kelly A, Hoshino Y, Hoshino S, Karulf MR, Rom WN, Weiden MD, Gold JA: Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS·One. 2009·Aug·12; 4(8):e6600. <·PMID:·19672303·>
• Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA: CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis. Am·J·Respir·Crit·Care·Med. 2008·Feb·1; 177(3):301‑8. <·PMID:·17989345·>
• Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR: TREM‑2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells. J·Immunol. 2008·Dec·1; 181(11):7863‑72. <·PMID:·19017976·>
• Steptoe RJ, Ritchie JM, Jones LK, Harrison LC: Autoimmune diabetes is suppressed by transfer of proinsulin‑encoding Gr‑1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes. 2005·Feb; 54(2):434‑42. <·PMID:·15677501·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN