特異性

The mouse monoclonal antibody ACR-2 recognizes various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

抗原情報

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod·Biol·Endocrinol. 2010·Jul·13; 8:87. <·PMID:·20626847·>
• Moos J, Peknicova J, Tesarik J: Protein‑protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol·Reprod. 1993·Aug; 49(2):408‑15. <·PMID:·8373968·>
• Moos J, Peknicova J, Tesarik J: Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore‑induced acrosome reaction. Biochim·Biophys·Acta. 1993·Apr·16; 1176(3):199‑207. <·PMID:·8471622·>
• Moos J, Tesarik J, Leca G, Peknicova J: Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS·Lett. 1991·Dec·2; 294(1‑2):27‑30. <·PMID:·1743288·>
• Peknicová J, et al.: Anim·Reprod·Sci. 1994; 35:255. <·No·PMID·>
• Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M: Monoclonal antibodies to intra‑acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001·Jul·15; 56(2):211‑23. <·PMID:·11480614·>
• Peknicova J, Moos J: Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990·Sep-Oct; 22(5):427‑35. <·PMID:·2073053·>