CyFlow CD324 FITC
品番 | CL261536 | ||
---|---|---|---|
抗体名 | Anti-Hu CD324 FITC,67A4 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 2 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
反応性|交差吸着 | Human | レーザー | Blue |
抗原 | CD324, E-Cadherin, cadherin1 | 最大蛍光波長 | 518 nm |
クローン | 67A4 | 最大励起波長 | 490 to 495 nm |
ホスト | Mouse | 標識/Format | FITC |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD324 FITC,67A4
特異性
The mouse monoclonal antibody 67A4 recognizes CD324 (E-cadherin) antigen, a 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
抗原情報
CD324 (E-cadherin) is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 (E-cadherin) is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 (E-cadherin)-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Armeanu S, Bühring HJ, Reuss-Borst M, Müller CA, Klein G: E‑cadherin is functionally involved in the maturation of the erythroid lineage. J·Cell·Biol. 1995·Oct; 131(1):243‑9. <·PMID:·7559781·>
• Bühring HJ, Müller T, Herbst R, Cole S, Rappold I, Schuller W, Zhu X, Fritzsch U, Faul C, Armeanu S, Ullrich A, Klein G, Schmidt H: The adhesion molecule E‑cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages. Leukemia. 1996·Jan; 10(1):106‑1. <·PMID:·8558914·>
• Caberg JH, Hubert PM, Begon DY, Herfs MF, Roncarati PJ, Boniver JJ, Delvenne PO: Silencing of E7 oncogene restores functional E‑cadherin expression in human papillomavirus 16‑transformed keratinocytes. Carcinogenesis. 2008·Jul; 29(7):1441‑7. <·PMID:·18566017·>
• Furio L, Guezennec A, Ducarre B, Guesnet J, Peguet-Navarro J: Differential effects of allergens and irritants on early differentiating monocyte‑derived dendritic cells. Eur·J·Dermatol. 2008·Mar-Apr; 18(2):141‑7. <·PMID:·18424372·>
• Kutlesa S, Wessels JT, Speiser A, Steiert I, Müller CA, Klein G: E‑cadherin‑mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation. J·Cell·Sci. 2002·Dec·1; 115(23):4505‑15. <·PMID:·12414996·>
• Lin JC, Liao SK, Lee EH, Hung MS, Sayion Y, Chen HC, Kang CC, Huang LS, Cherng JM: Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein‑Barr virus genome. J·Biomed·Sci. 2009·Nov·24; 16:105. <·PMID:·19930697·>
• Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T: A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). J·Invest·Dermatol. 2002·Oct; 119(4):842‑9. <·PMID:·12406329·>
• Pece S, Chiariello M, Murga C, Gutkind JS: Activation of the protein kinase Akt/PKB by the formation of E‑cadherin‑mediated cell‑cell junctions: Evidence for the association of phosphatidylinositol 3‑kinase with the E‑cadherin adhesion complex. J·Biol·Chem. 1999·Jul·2; 274(27):19347‑51. <·PMID:·10383446·>
• Pece S, Gutkind JS: Signaling from E‑cadherins to the MAPK pathway by the recruitment and activation of epidermal growth factor receptors upon cell‑cell contact formation. J·Biol·Chem. 2000·Dec·29; 275(52):41227‑33. <·PMID:·10969083·>
• Robertson H, Ali S, McDonnell BJ, Burt AD, Kirby JA: Chronic renal allograft dysfunction: the role of T cell‑mediated tubular epithelial to mesenchymal cell transition. J·Am·Soc·Nephrol. 2004·Feb; 15(2):390‑7. <·PMID:·14747385·>
• Servet-Delprat C, Vidalain PO, Bausinger H, Manié S, Le Deist F, Azocar O, Hanau D, Fischer A, Rabourdin-Combe C: Measles virus induces abnormal differentiation of CD40 ligand‑activated human dendritic cells. J·Immunol. 2000·Feb·15; 164(4):1753‑60. <·PMID:·10657621·>
• Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991·Mar·22; 251(5000):1451‑5. <·PMID:·2006419·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN