CyFlow CD300a PE
| 品番 | CK802473 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD300A PE,MEM-260 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD300a, IRC1, MAIR-I | 最大蛍光波長 | 576 nm |
| クローン | MEM-260 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD300A PE,MEM-260
特異性
The mouse monoclonal antibody MEM-260 recognizes CD300a antigen, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cels.
抗原情報
CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Alvarez Y, Tang X, Coligan JE, Borrego F: The CD300a (IRp60) inhibitory receptor is rapidly up‑regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling. Mol·Immunol. 2008·Jan; 45(1):253‑8. <·PMID:·17588661·>
• Bachelet I, Munitz A, Levi-Schaffer F: Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a. J·Allergy·Clin·Immunol. 2006·Jun; 117(6):1314‑20. <·PMID:·16750992·>
• Bachelet I, Munitz A, Moretta A, Moretta L, Levi-Schaffer F: The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells. J·Immunol. 2005·Dec·15; 175(12):7989‑95. <·PMID:·16339535·>
• Cantoni C, Bottino C, Augugliaro R, Morelli L, Marcenaro E, Castriconi R, Vitale M, Pende D, Sivori S, Millo R, Biassoni R, Moretta L, Moretta A: Molecular and functional characterization of IRp60, a member of the immunoglobulin superfamily that functions as an inhibitory receptor in human NK cells. Eur·J·Immunol. 1999·Oct; 29(10):3148‑59. <·PMID:·10540326·>
• Dimasi N, Roessle M, Moran O, Candiano G, Svergun DI, Biassoni R: Molecular analysis and solution structure from small‑angle X‑ray scattering of the human natural killer inhibitory receptor IRp60 (CD300a). Int·J·Biol·Macromol. 2007·Feb·20; 40(3):193‑200. <·PMID:·16949664·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN