特異性

The mouse monoclonal antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1) antigen, but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

抗原情報

利用方法

The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H, Geraghty DE: Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA‑E, F and G in Maternal‑Placental Immune Recognitions. J·Immunol. 2003; 171:1376. <·PMID:·12874228·>
• Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA‑G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003; 50(5):331‑8. <·PMID:·14628085·>
• Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA‑G expression in malignant hematopoetic cells from leukemia patients. Leuk·Res. 2003·Jul; 27(7):643‑8. <·PMID:·12681364·>
• Polakova K, Russ G: Expression of the non‑classical HLA‑G antigen in tumor cell lines is extremely restricted. Neoplasma. 2000; 47(6):342‑8. <·PMID:·11263857·>
• Real LM, Cabrera T, Canton J, Oliva R, Ruiz-Cabello F, Garrido F: Looking for HLA‑G expression in human tumours. J·Reprod·Immunol. 1999·Jul; 43(2):263‑73. <·PMID:·10479062·>
• Real LM, Cabrera T, Collado A, Jimenez P, Garcia A, Ruiz-Cabello F, Garrido F: Expression of HLA G in human tumors is not a frequent event. Int·J·Cancer. 1999·May·17; 81(4):512‑8. <·PMID:·10225437·>
• Wiendl H, Feger U, Mittelbronn M, Jack C, Schreiner B, Stadelmann C, Antel J, Brueck W, Meyermann R, Bar-Or A, Kieseier BC, Weller M: Expression of the immune‑tolerogenic major histocompatibility molecule HLA‑G in multiple sclerosis: implications for CNS immunity. Brain. 2005·Nov; 128(11):2689‑2704. <·PMID:·16123145·>