CyFlow CD158f PE
|抗体名||Anti-Hu CD158f PE,UP-R1|
| Mouse IgG1 PE (BV655324)
|反応性|交差吸着||Human||レーザー||Blue, Green, Yellow|
|抗原||CD158f, KIR2DL5||最大蛍光波長||576 nm|
|クローン||UP-R1||最大励起波長||496 nm, 565 nm|
Anti-Hu CD158f PE,UP-R1
The mouse monoclonal antibody UP-R1 recognizes CD158f antigen, a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.
CD158f (KIR2DL5) is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Do not use after expiration date stamped on vial label.
• Du Z, Sharma SK, Spellman S, Reed EF, Rajalingam R: KIR2DL5 alleles mark certain combination of activating KIR genes. Genes·Immun. 2008·Jul; 9(5):470‑80. <·PMID:·18509341·>
• Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J·Immunol. 2007·Apr·1; 178(7):4402‑10. <·PMID:·17371997·>
• Yusa S, Catina TL, Campbell KS: KIR2DL5 can inhibit human NK cell activation via recruitment of Src homology region 2‑containing protein tyrosine phosphatase‑2 (SHP‑2). J·Immunol. 2004·Jun·15; 172(12):7385‑92. <·PMID:·15187115·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN