CyFlow CD45R FITC
| 品番 | BY978670 | ||
|---|---|---|---|
| 抗体名 | Anti-H/M CD45R FITC,RA3-6B2 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Human | Mouse | Cat | レーザー | Blue |
| 抗原 | CD45R | 最大蛍光波長 | 518 nm |
| クローン | RA3-6B2 | 最大励起波長 | 490 to 495 nm |
| ホスト | Rat | 標識/Format | FITC |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping | Phosphorylation |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-H/M CD45R FITC,RA3-6B2
特異性
The rat monoclonal antibody RA3-6B2 recognizes an epitope on CD45R antigen, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
抗原情報
CD45R (B220) is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
利用方法
The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220‑kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J·Immunol. 2004·Apr·1; 172(7):4298‑307. <·PMID:·15034044·>
• Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi‑infected joint tissue. J·Immunol. 2012·Sep·1; 189(5):2488‑501. <·PMID:·22851707·>
• Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B‑1 B cell‑specified progenitor. Nat·Immunol. 2006·Mar; 7(3):293‑301. <·PMID:·16429139·>
• Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2‑deficient mice suggests two stages of mutational targeting. Immunity. 1998·Jul; 9(1):135‑41. <·PMID:·9697843·>
• Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry·A. 2006·Apr; 69(4):249‑59. <·PMID:·16538653·>
• Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven‑color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J·Histochem·Cytochem. 2000·May; 48(5):653‑62. <·PMID:·10769049·>
• Watanabe Y, Akaike T: Activation signal induces the expression of B cell‑specific CD45R epitope (6B2) on murine T cells. Scand·J·Immunol. 1994·May; 39(5):419‑25. <·PMID:·7514808·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN