CyFlow CD134 PE
品番 | BV467980 | ||
---|---|---|---|
抗体名 | Anti-Hu CD134 PE,Ber-ACT35 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
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反応性|交差吸着 | Human | Non-Human Primates | レーザー | Blue, Green, Yellow |
抗原 | CD134, OX40, TNFRSF4 | 最大蛍光波長 | 576 nm |
クローン | Ber-ACT35 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD134 PE,Ber-ACT35
特異性
The mouse monoclonal antibody Ber-ACT35 (also known as ACT35) recognizes CD134 antigen, a 50 kDa type I transmembrane glycoprotein expressed on activated T cells.
抗原情報
CD134 (TNFRSF4, OX40) is a type I transmembrane glycoprotein of TNF/NGF receptor family expressed on activated T cells, fibroblasts, and hematopoietic precursors. Binding to its ligand (OX40L, TNFSF4) on antigen presenting cells gives to the T cell costimulatory signal, and this interaction results also in B cell proliferation and influences T cell memory pool. CD134 is upregulated at sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Aten J, Roos A, Claessen N, Schilder-Tol EJ, Ten Berge IJ, Weening JJ: Strong and selective glomerular localization of CD134 ligand and TNF receptor‑1 in proliferative lupus nephritis. J·Am·Soc·Nephrol. 2000·Aug; 11(8):1426‑38. <·PMID:·10906156·>
• Endl J, Rosinger S, Schwarz B, Friedrich SO, Rothe G, Karges W, Schlosser M, Eiermann T, Schendel DJ, Boehm BO: Coexpression of CD25 and OX40 (CD134) receptors delineates autoreactive T‑cells in type 1 diabetes. Diabetes. 2006·Jan; 55(1):50‑60. <·PMID:·16380476·>
• Jones D, Fletcher CD, Pulford K, Shahsafaei A, Dorfman DM: The T‑cell activation markers CD30 and OX40/CD134 are expressed in nonoverlapping subsets of peripheral T‑cell lymphoma. Blood. 1999·May·15; 93(10):3487‑93. <·PMID:·10233901·>
• Li M, Zhang Y: The effect of anti‑human CD134 monoclonal antibody on phytohemagglutinin‑induced mRNA expression of perforin in peripheral blood mononuclear cells. Cell·Mol·Immunol. 2005·Dec; 2(6):467‑71. <·PMID:·16426498·>
• Tanijiri T, Shimizu T, Uehira K, Yokoi T, Amuro H, Sugimoto H, Torii Y, Tajima K, Ito T, Amakawa R, Fukuhara S: Hodgkin's reed‑sternberg cell line (KM‑H2) promotes a bidirectional differentiation of CD4+CD25+Foxp3+ T cells and CD4+ cytotoxic T lymphocytes from CD4+ naive T cells. J·Leukoc·Biol. 2007·Sep; 82(3):576‑84. <·PMID:·17545218·>
• Voo KS, Bover L, Harline ML, Vien LT, Facchinetti V, Arima K, Kwak LW, Liu YJ: Antibodies targeting human OX40 expand effector T cells and block inducible and natural regulatory T cell function. J·Immunol. 2013·Oct·1; 191(7):3641‑50. <·PMID:·24014877·>
• Willett BJ, McMonagle EL, Ridha S, Hosie MJ: Differential utilization of CD134 as a functional receptor by diverse strains of feline immunodeficiency virus. J·Virol. 2006·Apr; 80(7):3386‑94. <·PMID:·16537606·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN