CyFlow ARHGEF4 PE
品番 | BV459562 | ||
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抗体名 | Anti-Hu ARHGEF4 PE,ARHGEF-08 | ||
包装単位 | 0.1 mg | ||
濃度 | 0.1 mg/ml | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
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反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
抗原 | ARHGEF4 | 最大蛍光波長 | 576 nm |
クローン | ARHGEF-08 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgG1 | 研究分野 | Signaling |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu ARHGEF4 PE,ARHGEF-08
特異性
The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4, a 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.
抗原情報
ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF1 (Adenomatous polyposis coli - stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
利用方法
The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3‑kinase. J·Biol·Chem. 2009·Aug·14; 284(33):22436‑43. <·PMID:·19525225·>
• Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL‑AML1 to promote leukaemic transformation. Leuk·Res. 2010·Jan; 34(1):109‑15. <·PMID:·19628279·>
• Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell·Res. 2012·Feb; 22(2):372‑86. <·PMID:·21788986·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN