CyFlow CD54 PerCP
| 品番 | BN093653 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD54 PERCP,1H4 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2b PerCP (BE922304) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green |
| 抗原 | CD54, ICAM-1 | 最大蛍光波長 | 678 nm |
| クローン | 1H4 | 最大励起波長 | 482 to 490 nm |
| ホスト | Mouse | 標識/Format | PerCP |
| アイソタイプ | IgG2b | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD54 PERCP,1H4
特異性
The mouse monoclonal antibody 1H4 recognizes CD54 antigen, a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
抗原情報
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM‑1) during lymphohematopoietic differentiation. Blood. 1989·May·15; 73(7):1896‑903. <·PMID:·2469503·>
• Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM‑1) has a central role in cell‑cell contact‑mediated immune mechanisms. Proc·Natl·Acad·Sci·USA. 1988·May; 85(9):3095‑9. <·PMID:·3362863·>
• Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum‑infected erythrocytes bind ICAM‑1 at a site distinct from LFA‑1, Mac‑1, and human rhinovirus. Cell. 1992·Jan·10; 68(1):63‑9. <·PMID:·1346257·>
• Springer TA: Adhesion receptors of the immune system. Nature. 1990·Aug·2; 346(6283):425‑34. <·PMID:·1974032·>
• Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay‑accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J·Gen·Virol. 2004·Mar; 85(3):731‑8. <·PMID:·14993659·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN