CyFlow CD146 PE
品番 | BG312271 | ||
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抗体名 | Anti-H/M CD146 PE,P1H12 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
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反応性|交差吸着 | Human | Mouse | Dog | Rabbit | レーザー | Blue, Green, Yellow |
抗原 | CD146, MCAM | 最大蛍光波長 | 576 nm |
クローン | P1H12 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-H/M CD146 PE,P1H12
特異性
The mouse monoclonal antibody P1H12 recognizes CD146 antigen, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.
抗原情報
CD146 (MCAM; melanoma cell adhesion molecule or MUC18) is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Finney MR, Greco NJ, Haynesworth SE, Martin JM, Hedrick DP, Swan JZ, Winter DG, Kadereit S, Joseph ME, Fu P, Pompili VJ, Laughlin MJ: Direct comparison of umbilical cord blood versus bone marrow‑derived endothelial precursor cells in mediating neovascularization in response to vascularischemia. Biol·Blood·Marrow·Transplant. 2006·May; 12(5):585‑93. <·PMID:·16635794·>
• Kamstock D, Guth A, Elmslie R, Kurzman I, Liggitt D, Coro L, Fairman J, Dow S: Liposome‑DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma. Cancer·Gene·Ther. 2006·Mar; 13(3):306‑17. <·PMID:·16138118·>
• Solovey A, Lin Y, Browne P, Choong S, Wayner E, Hebbel RP: Circulating activated endothelial cells in sickle cell anemia. N·Engl·J·Med. 1997·Nov·27; 337(22):1584‑90. <·PMID:·9371854·>
• Solovey AN, Gui L, Chang L, Enenstein J, Browne PV, Hebbel RP: Identification and functional assessment of endothelial P1H12. J·Lab·Clin·Med. 2001·Nov; 138(5):322‑31. <·PMID:·11709656·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN