CyFlow STRO1 PE
品番 | BA518386 | ||
---|---|---|---|
抗体名 | Anti-Hu STRO1 PE,STRO-1 | ||
包装単位 | 0.1 mg | ||
濃度 | 0.1 mg/ml | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
- | ||
反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
抗原 | STRO1 | 最大蛍光波長 | 576 nm |
クローン | STRO-1 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgM | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu STRO1 PE,STRO-1
特異性
The mouse monoclonal antibody STRO-1 recognizes the cell surface STRO1 antigen expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
抗原情報
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
利用方法
The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bensidhoum M, Chapel A, Francois S, Demarquay C, Mazurier C, Fouillard L, Bouchet S, Bertho JM, Gourmelon P, Aigueperse J, Charbord P, Gorin NC, Thierry D, Lopez M: Homing of in vitro expanded Stro‑1‑ or Stro‑1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment. Blood. 2004·May·1; 103(9):3313‑9. <·PMID:·14715641·>
• Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ: Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J·Cell·Sci. 2003·May·1; 116(9):1827‑35. <·PMID:·12665563·>
• Kaneko R, Akita H, Shimauchi H, Sasano Y: Immunohistochemical localization of the STRO‑1 antigen in developing rat teeth by light microscopy and electron microscopy. J·Electron·Microsc·(Tokyo). 2009·Dec; 58(6):363‑73. <·PMID:·19525367·>
• Oyajobi BO, Lomri A, Hott M, Marie PJ: Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO‑1 monoclonal antibody. J·Bone·Miner·Res. 1999·Mar; 14(3):351‑61. <·PMID:·10027900·>
• Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S: Recovery of stem cells from cryopreserved periodontal ligament. J·Dent·Res. 2005·Oct; 84(10):907‑12. <·PMID:·16183789·>
• Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO‑1. Blood. 1991·Jul·1; 78(1):55‑62. <·PMID:·2070060·>
• Stenderup K, Justesen J, Eriksen EF, Rattan SI, Kassem M: Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J·Bone·Miner·Res. 2001·Jun; 16(6):1120‑9. <·PMID:·11393789·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN