特異性

The mouse monoclonal antibody G233 recognizes several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHC Class Ib). The antibody G233 has been found not to cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).

抗原情報

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Blaschitz A, Hutter H, Dohr G: HLA Class I protein expression in the human placenta. Early·Pregnancy. 2001·Jan; 5(1):67‑9. <·PMID:·11753519·>
• Bulmer JN, Cioni R, Bussani C, Cirigliano V, Sole F, Costa C, Garcia P, Adinolfi M: HLA‑G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF‑PCR. Prenat·Diagn. 2003·Jan; 23(1):34‑9. <·PMID:·12533810·>
• Frumento G, Franchello S, Palmisano GL, Nicotra MR, Giacomini P, Loke YW, Geraghty DE, Maio M, Manzo C, Natali PG, Ferrara GB: Melanomas and melanoma cell lines do not express HLA‑G, and the expression cannot be induced by gammaIFN treatment. Tissue·Antigens. 2000·Jul; 56(1):42581. <·PMID:·10958353·>
• Gonzalez A, Alegre E, Arroyo A, LeMaoult J, Echeveste JI: Identification of circulating nonclassic human leukocyte antigen G (HLA‑G)‑like molecules in exudates. Clin·Chem. 2011·Jul; 57(7):1013‑22. <·PMID:·21527645·>
• Imudia AN, Suzuki Y, Kilburn BA, Yelian FD, Diamond MP, Romero R, Armant DR: Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study. Hum·Reprod. 2009·Sep; 24(9):2086‑92. <·PMID:·19497946·>
• Loke YW, King A, Burrows T, Gardner L, Bowen M, Hiby S, Howlett S, Holmes N, Jacobs D: Evaluation of trophoblast HLA‑G antigen with a specific monoclonal antibody. Tissue·Antigens. 1997·Aug; 50(2):135‑46. <·PMID:·9271823·>
• Mandelboim O, Pazmany L, Davis DM, Vales-Gomez M, Reyburn HT, Rybalov B, Strominger JL: Multiple receptors for HLA‑G on human natural killer cells. Proc·Natl·Acad·Sci·USA. 1997·Dec·23; 94(26):14666‑70. <·PMID:·9405670·>
• Rajagopalan S, Long EO: A human histocompatibility leukocyte antigen (HLA)‑G‑specific receptor expressed on all natural killer cells. J·Exp·Med. 1999·Apr·5; 189(7):1093‑100. <·PMID:·10190900·>
• Van Lierop MJ, Wijnands F, Loke YW, Emmer PM, Lukassen HG, Braat DD, van der Meer A, Mosselman S, Joosten I: Detection of HLA‑G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B. Mol·Hum·Reprod. 2002·Aug; 8(8):776‑84. <·PMID:·12149411·>