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CyFlow CD79a PE

品番 AX816454
抗体名 Anti-H/M/R CD79A PE,HM57
包装単位 100 tests
濃度 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human | Mouse | Rat | Pig | Cow | Horse | Rabbit | Guinea pig | Opossum | Chicken レーザー Blue, Green, Yellow
抗原 CD79a, IgA, MB-1, MB1 最大蛍光波長 576 nm
クローン HM57 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-H/M/R CD79A PE,HM57

特異性

The mouse monoclonal antibody HM57 recognizes CD79a, a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

抗原情報

CD79a (Ig α, MB1) forms disulfide-linked heterodimer with CD79b (Ig β, B29). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with λ5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand‑independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J·Exp·Med. 2001·Dec·3; 194(11):1583‑96. <·PMID:·11733573·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross‑reactive anti‑human monoclonal antibodies as a tool for B‑cell identification in dogs and pigs. Vet·Immunol·Immunopathol. 2007·Sep·15; 119(1‑2):56‑62. <·PMID:·17673300·>
• Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a)‑ and Igbeta (CD79b)‑mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J·Immunol. 2006·Dec·1; 177(11):7913‑22. <·PMID:·17114463·>
• Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre‑B cell antigen receptor‑dependent processes. J·Immunol. 2004·Jul·15; 173(2):1000‑11. <·PMID:·15240688·>
• Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J·Immunol. 2005·Feb·1; 174(3):1245‑52. <·PMID:·15661879·>
• Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY: Detection of T and B cells in many animal species using cross‑reactive anti‑peptide antibodies. J·Immunol. 1993·Jun·15; 150(12):5429‑35. <·PMID:·8515069·>
• Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al.: CD79a: a novel marker for B‑cell neoplasms in routinely processed tissue samples. Blood. 1995·Aug·15; 86(4):1453‑9. <·PMID:·7632952·>
• Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al: The IgM‑associated protein mb‑1 as a marker of normal and neoplastic B cells. J·Immunol. 1991·Dec·1; 147(11):2474‑82. <·PMID:·1747162·>
• Mason DY, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ: The B29 and mb‑1 polypeptides are differentially expressed during human B cell differentiation. Eur·J·Immunol. 1992·Oct; 22(10):2753‑6. <·PMID:·1396979·>
• Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J·Immunol. 2004·Feb·15; 172(4):2210‑8. <·PMID:·14764688·>
• van Noesel CJ, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J: The membrane IgM‑associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb‑1 gene. J·Immunol. 1991·Jun·1; 146(11):3881‑8. <·PMID:·2033258·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD79a PE (HM57) AX816454 TDS (US)