特異性

The rat monoclonal antibody M1/70 recognizes CD11b antigen, a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

抗原情報

CD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Ault KA, Springer TA: Cross‑reaction of a rat‑anti‑mouse phagocyte‑specific monoclonal antibody (anti‑Mac‑{01} with human monocytes and natural killer cells. J·Immunol. 1981·Jan; 126(1):359‑64. <·PMID:·7451976·>
• Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood‑borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J·Appl·Physiol. 2003·Sep; 95(3):969‑76. <·PMID:·12730143·>
• Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor‑specific antigen (idiotype) and activate CD4+ T cells. Proc·Natl·Acad·Sci·USA. 2000·Mar·14; 97(6):2697‑702. <·PMID:·10706628·>
• Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J·Immunol. 2006·Nov·15; 177(10):6974‑82. <·PMID:·17082612·>
• Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha‑ and beta‑subunits of two related mouse glycoproteins involved in cell interactions, LFA‑1 and Mac‑1. J·Exp·Med. 1983·Aug·1; 158(2):586‑602. <·PMID:·6193226·>
• Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur·J·Immunol. 1978·Aug; 8(8):539‑51. <·PMID:·81133·>
• Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM‑3 grabbing nonintegrin‑related 1 (SIGNR{01} and complement receptor type 3 (CR3) in the uptake of oligomannose‑coated liposomes by macrophages. Glycobiology. 2009·Mar; 19(3):258‑66. <·PMID:·19029201·>
• Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon‑injured arteries. Arterioscler·Thromb·Vasc·Biol. 2000·Dec; 20(12):2553‑8. <·PMID:·11116052·>
• Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T‑cell subsets and other leukocytes in paraffin‑embedded rat and mouse tissues with monoclonal antibodies. J·Histochem·Cytochem. 1995·Mar; 43(3):313‑20. <·PMID:·7868861·>
• Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft‑versus‑host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J·Immunol. 2002·Mar·15; 168(6):3088‑98. <·PMID:·11884483·>