特異性

The mouse monoclonal antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its β2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).

抗原情報

利用方法

The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 1 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA‑G monoclonal antibodies to soluble HLA‑G molecules. Tissue·Antigens. 2000·Jun; 55(6):510‑8. <·PMID:·10902607·>
• Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR: HLA‑G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur·J·Immunol. 2002·Feb; 32(2):311‑5. <·PMID:·11807769·>
• Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor‑1 distinguishes between conformed and beta 2‑microglobulin‑free HLA‑G molecules. J·Immunol. 2005·Oct·15; 175(8):4866‑74. <·PMID:·16210588·>
• Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A: Regulatory role of tryptophan degradation pathway in HLA‑G expression by human monocyte‑derived dendritic cells. Mol·Immunol. 2006·Jul; 43(14):2151‑60. <·PMID:·16490253·>
• Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J: Monocytes and T lymphocytes in HIV‑1‑positive patients express HLA‑G molecule. AIDS. 2002·Feb·15; 16(3):347‑51. <·PMID:·11834945·>
• Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·>
• Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R: Lung macrophages and dendritic cells express HLA‑G molecules in pulmonarydiseases. Hum·Immunol. 2002·Feb; 63(2):83‑90. <·PMID:·11821155·>
• Zhao L, Teklemariam T, Hantash BM: Reassessment of HLA‑G isoform specificity of MEM‑G/9 and 4H84 monoclonal antibodies. Tissue·Antigens. 2012·Sep; 80(3):231‑8. <·PMID:·22738368·>
• Abstracts from the 3rd International Conference on HLA‑G; Paris, July 2003. Tissue·Antigens. 2003; 62(4):340‑57. <·Wiley·j.1399-0039·>