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CyFlow CD59 Purified

品番 AS570823
抗体名 Anti-Hu CD59 PURI,MEM-43
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG2a Purified (CJ747338)
Mouse IgG2a Purified (AP005692)
反応性|交差吸着 Human レーザー
抗原 CD59, Protectin H19 最大蛍光波長 -
クローン MEM-43 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG2a 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Immunohistochmistry (paraffin-embedded sections)

Anti-Hu CD59 PURI,MEM-43

特異性

The mouse monoclonal antibody MEM-43 recognizes well defined epitope (W40, R-53) on CD59 antigen, an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.

抗原情報

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Awasthi S, Wolf R, White G: Ontogeny and phagocytic function of baboon lung dendritic cells. Immunol·Cell·Biol. 2009·Jul; 87(5):419‑27. <·PMID:·19223853·>
• Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement‑inhibitory glycoprotein, CD59. J·Exp·Med. 1997·Feb·3; 185(3):507‑16. <·PMID:·9053451·>
• Cebecauer M, Cerny J, Horejsi V: Incorporation of leucocyte GPI‑anchored proteins and protein tyrosine kinases into lipid‑rich membrane domains of COS‑7 cells. Biochem·Biophys·Res·Commun. 1998·Feb·24; 243(3):706‑10. <·PMID:·9500981·>
• Cinek T, Horejsi V: The nature of large noncovalent complexes containing glycosyl‑phosphatidylinositol‑anchored membrane glycoproteins and protein tyrosine kinases.. J·Immunol. 1992·Oct·1; 149(7):2262‑70. <·PMID:·1382093·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Forsberg UH, Bazil V, Stefanova I, Schroder J: Gene for human CD59 (likely Ly‑6 homologue) is located on the short arm of chromosome 11. Immunogenetics. 1989; 30(3):188‑93. <·PMID:·2476389·>
• Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia·Biol·(Praha). 1986; 32(1):12‑25. <·PMID:·2422063·>
• Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc·Natl·Acad·Sci·USA. 2007·Mar·20; 104(12):5062‑6. <·PMID:·17360380·>
• Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·>
• Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J·Immunol. 2007·Feb·15; 178(4):2262‑71. <·PMID:·17277131·>
• Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP: Expression of glycosylphosphatidylinositol‑anchored CD59 on target cells enhances human NK cell‑mediated cytotoxicity. J·Immunol. 2006·Mar·1; 176(5):2915‑23. <·PMID:·16493049·>
• Sutherland DR, Keeney M, Illingworth A: Practical guidelines for the high‑sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry. Cytometry·B·Clin·Cytom. 2012·Jul; 82(4):195‑208. <·PMID:·22499484·>
• Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J·Immunol. 2005·Jun·1; 174(11):7033‑42. <·PMID:·15905546·>
• Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM‑43 anchored in membrane through phosphatidylinositol. Mol·Immunol. 1989·Feb; 26(2):153‑61. <·PMID:·2918859·>
• Stefanova I, Horejsi V, Ansotegui IJ, Knapp W, Stockinger H: GPI‑anchored cell‑surface molecules complexed to protein tyrosine kinases. Science. 1991·Nov·15; 254(5034):1016‑9. <·PMID:·1719635·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD59 Purified (MEM-43) AS570823 TDS (US)