CyFlow CD28 PE
| 品番 | AR813910 | ||
|---|---|---|---|
| 抗体名 | Anti-Rt CD28 PE,JJ319 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Rat | レーザー | Blue, Green, Yellow |
| 抗原 | CD28, Tp44 | 最大蛍光波長 | 576 nm |
| クローン | JJ319 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Rt CD28 PE,JJ319
特異性
The mouse monoclonal antibody JJ319 recognizes rat CD28 antigen, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
抗原情報
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. Its analog CTLA-4, expressed on the surface of activated T cells, has higher affinity to CD80 and CD86 than CD28 and downregulates T cell activity.
利用方法
The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Dengler TJ, Szabo G, Sido B, Nottmeyer W, Zimmerman R, Vahl CF, Hünig T, Meuer SC: Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high‑responder rat heart transplantation. Transplantation. 1999·Feb·15; 67(3):392‑8. <·PMID:·10030284·>
• Guillonneau C, Séveno C, Dugast AS, Li XL, Renaudin K, Haspot F, Usal C, Veziers J, Anegon I, Vanhove B: Anti‑CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig‑treated heart allograft recipients. J·Immunol. 2007·Dec·15; 179(12):8164‑71. <·PMID:·18056359·>
• Haspot F, Villemain F, Laflamme G, Coulon F, Olive D, Tiollier J, Soulillou JP, Vanhove B: Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self‑restricted responses. Blood. 2002·Mar·15; 99(6):2228‑34. <·PMID:·11877302·>
• Kerstan A, Armbruster N, Leverkus M, Hünig T: Cyclosporin A abolishes CD28‑mediated resistance to CD95‑induced apoptosis via superinduction of caspase‑3. J·Immunol. 2006·Dec·1; 177(11):7689‑97. <·PMID:·17114439·>
• Laskowski IA, Pratschke J, Wilhelm MJ, Dong VM, Beato F, Taal M, Gasser M, Hancock WW, Sayegh MH, Tilney NL: Anti‑CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats. J·Am·Soc·Nephrol. 2002·Feb; 13(2):519‑27. <·PMID:·11805183·>
• Lühder F, Huang Y, Dennehy KM, Guntermann C, Müller I, Winkler E, Kerkau T, Ikemizu S, Davis SJ, Hanke T, Hünig T: Topological requirements and signaling properties of T cell‑activating, anti‑CD28 antibody superagonists. J·Exp·Med. 2003·Apr·21; 197(8):955‑66. <·PMID:·12707299·>
• Robinson LE, Clandinin MT, Field CJ: R3230AC rat mammary tumor and dietary long‑chain (n‑3) fatty acids change immune cell composition and function during mitogen activation. J·Nutr. 2001·Jul; 131(7):2021‑7. <·PMID:·11435524·>
• Tacke M, Hanke G, Hanke T, Hünig T: CD28‑mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur·J·Immunol. 1997·Jan; 27(1):239‑47. <·PMID:·9022025·>
• van den Brandt J, Wang D, Reichardt HM: Resistance of single‑positive thymocytes to glucocorticoid‑induced apoptosis is mediated by CD28 signaling. Mol·Endocrinol. 2004·Mar; 18(3):687‑95. <·PMID:·14701943·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN