CyFlow Beta3-tubulin FITC
| 品番 | AR041515 | ||
|---|---|---|---|
| 抗体名 | BETA3-TUBULIN FITC,TU-20 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Broad Spectrum | レーザー | Blue |
| 抗原 | β3-tubulin | 最大蛍光波長 | 518 nm |
| クローン | TU-20 | 最大励起波長 | 490 to 495 nm |
| ホスト | Mouse | 標識/Format | FITC |
| アイソタイプ | IgG1 | 研究分野 | Cytoskeleton | Neurobiology |
| クローナリティ | monoclonal | 用途 | Immunocytochemistry |
BETA3-TUBULIN FITC,TU-20
特異性
The mouse monoclonal antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human β3-tubulin.
抗原情報
The β3-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumors originating from these cells. The β3-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
利用方法
The reagent is designed for Immunocytochemistry. Suggested working usage is 2.5 µg/ml. The conjugate was also successfully used on paraffin sections using confocal microscopy. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Draberova E, Lukas Z, Ivanyi D, Viklicky V, Draber P: Expression of class III beta‑tubulin in normal and neoplastic human tissues.. Histochem·Cell·Biol. 1998·Mar; 109(3):231‑9. <·PMID:·9541471·>
• Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V: Expression of class III beta‑tubulin in colorectal carcinomas: an immunohistochemical study using TU‑20&TuJ‑1 antibody. Indian·J·Med·Res. 2009·Jan; 129(1):89‑94. <·PMID:·19287064·>
• Jirasek T, Pisarikova E, Viklicky V, Mandys V: Expression of class III beta‑tubulin in malignant epithelial tumours: an immunohistochemical study using TU‑20 and TuJ‑1 antibodies. Folia·Histochem·Cytobiol. 2007; 45(1):41‑5. <·PMID:·17378245·>
• Katsetos CD, Dráberová E, Smejkalová B, Reddy G, Bertrand L, de Chadarévian J-P, Legido A, Nissanov J, Baas PW, Dráber P: Class III beta‑tubulin and gamma‑tubulin are co‑expressed and form complexes in human glioblastoma cells.. Neurochem·Res. 2007; 32:1387‑1398. <·PMID:·20162618·>
• Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma‑tubulin with nonreceptor protein tyrosine kinases Src and Fynin differentiating P19 embryonal carcinoma cells. Exp·Cell·Res. 2004·Aug·1; 298(1):218‑28. <·PMID:·15242776·>
• Peknicova J, Kubatova A, Sulimenko V, Draberova E, Viklicky V, Hozak P, Draber P: Differential subcellular distribution of tubulin epitopes in boar spermatozoa:recognition of class III beta‑tubulin epitope in sperm tail. Biol·Reprod. 2001·Sep; 65(3):672‑9. <·PMID:·11514327·>
• Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M: A high throughput embryonic stem cell screen identifies Oct‑2 as a bifunctional regulator of neuronal differentiation. Genes·Dev. 2009·Mar·1; 23(5):575‑88. <·PMID:·19270158·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN