CyFlow CD11b PE
品番 | AL816358 | ||
---|---|---|---|
抗体名 | Anti-Hu/Ms CD11B PE,M1/70 | ||
包装単位 | 0.1 mg | ||
濃度 | 0.5 mg/ml | ||
容量 | 0.2 ml | ||
関連製品 (アイソタイプコントロール) |
- | ||
反応性|交差吸着 | Human | Mouse | Non-Human Primates | Rabbit | レーザー | Blue, Green, Yellow |
抗原 | CD11b, Mac-1 | 最大蛍光波長 | 576 nm |
クローン | M1/70 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Rat | 標識/Format | PE |
アイソタイプ | IgG2b | 研究分野 | Immunophenotyping | MHC |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu/Ms CD11B PE,M1/70
特異性
The rat monoclonal antibody M1/70 recognizes CD11b antigen, a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
抗原情報
CD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 0.5 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Ault KA, Springer TA: Cross‑reaction of a rat‑anti‑mouse phagocyte‑specific monoclonal antibody (anti‑Mac‑{01} with human monocytes and natural killer cells. J·Immunol. 1981·Jan; 126(1):359‑64. <·PMID:·7451976·>
• Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood‑borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J·Appl·Physiol. 2003·Sep; 95(3):969‑76. <·PMID:·12730143·>
• Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor‑specific antigen (idiotype) and activate CD4+ T cells. Proc·Natl·Acad·Sci·USA. 2000·Mar·14; 97(6):2697‑702. <·PMID:·10706628·>
• Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J·Immunol. 2006·Nov·15; 177(10):6974‑82. <·PMID:·17082612·>
• Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha‑ and beta‑subunits of two related mouse glycoproteins involved in cell interactions, LFA‑1 and Mac‑1. J·Exp·Med. 1983·Aug·1; 158(2):586‑602. <·PMID:·6193226·>
• Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur·J·Immunol. 1978·Aug; 8(8):539‑51. <·PMID:·81133·>
• Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM‑3 grabbing nonintegrin‑related 1 (SIGNR{01} and complement receptor type 3 (CR3) in the uptake of oligomannose‑coated liposomes by macrophages. Glycobiology. 2009·Mar; 19(3):258‑66. <·PMID:·19029201·>
• Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon‑injured arteries. Arterioscler·Thromb·Vasc·Biol. 2000·Dec; 20(12):2553‑8. <·PMID:·11116052·>
• Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T‑cell subsets and other leukocytes in paraffin‑embedded rat and mouse tissues with monoclonal antibodies. J·Histochem·Cytochem. 1995·Mar; 43(3):313‑20. <·PMID:·7868861·>
• Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft‑versus‑host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J·Immunol. 2002·Mar·15; 168(6):3088‑98. <·PMID:·11884483·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN