CyFlow Ki-67 Alexa Fluor 647
品番 | AD268830 | ||
---|---|---|---|
抗体名 | Anti-Hu KI-67 AF 647,Ki-67 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 0.4 ml | ||
関連製品 (アイソタイプコントロール) |
- | ||
反応性|交差吸着 | Human | Cow | レーザー | Red |
抗原 | Ki-67 | 最大蛍光波長 | 665 nm |
クローン | Ki-67 | 最大励起波長 | 650 nm |
ホスト | Mouse | 標識/Format | Alexa Fluor™ 647 |
アイソタイプ | IgG1 | 研究分野 | Transcription |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu KI-67 AF 647,Ki-67
特異性
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
抗原情報
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation‑associated nuclear antigen defined by antibody Ki‑67: a new kind of cell‑cycle‑maintaining proteins. Arch·Immunol·Ther·Exp·(Warsz). 1995; 43(2):117‑21. <·PMID:·8744726·>
• Gerdes J: Ki‑67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin·Cancer·Biol. 1990·Jun; 1(3):199‑206. <·PMID:·2103495·>
• Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation‑associated human nuclear antigen defined by the monoclonal antibody Ki‑67. J·Immunol. 1984·Oct; 133(4):1710‑5. <·PMID:·6206131·>
• Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int·J·Cancer. 1983·Jan·15; 31(1):13‑20. <·PMID:·6339421·>
• Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation‑associated antigen of antibody Ki‑67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle‑maintaining proteins. J·Cell·Biol. 1993·Nov; 123(3):513‑22. <·PMID:·8227122·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN