特異性

The mouse monoclonal antibody SIT-01 recognizes SIT (SHP2-interacting transmembrane adaptor protein) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.

抗原情報

SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat·Rev·Immunol. 2004·Aug; 4(8):603‑16. <·PMID:·15286727·>
• Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2‑interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001·May-Jun; 53(4):337‑41. <·PMID:·11491537·>
• Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2‑interacting transmembrane adaptor protein (SIT), a novel disulfide‑linked dimer regulating human T cell activation. J·Exp·Med. 1999·Apr·19; 189(8):1181‑94. <·PMID:·10209036·>
• Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T‑cell functions. Mol·Cell·Biol. 2005·Sep; 25(17):7557‑68. <·PMID:·16107703·>
• Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid‑cell markers. Blood. 2006·Jan·1; 107(1):213‑21. <·PMID:·16160011·>