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CyFlow IgG1 PerCP-Cy5.5

品番 CZ886982
抗体名 Mouse IGG1 PERCP-CY5.5,MOPC-21
包装単位 0.1 mg
濃度 0.1 mg/ml
容量 1 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 レーザー Blue, Green
抗原 IgG1 Isotype Control 最大蛍光波長 695 nm
クローン MOPC-21 最大励起波長 482 nm
ホスト Mouse 標識/Format PerCP-Cy5.5
アイソタイプ IgG1 研究分野 Isotype controls
クローナリティ monoclonal 用途

Flow cytometry (Isotype control)

Mouse IGG1 PERCP-CY5.5,MOPC-21

特異性

This mouse IgG1 κ monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.

抗原情報

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

利用方法

The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10 µg/ml of incubation mixture), change the experimental conditions to reduce the background.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO: Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J·Exp·Med. 2005·Oct·3; 202(7):1001‑12. <·PMID:·16203869·>
• Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule‑1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer·Res. 2007·Feb·1; 67(3):1317‑25. <·PMID:·17283169·>
• Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor‑like phenotype in low‑grade glioma and enhanced CD133‑expression and neuronal lineage differentiation potential in high‑grade glioma. PLoS·One. 2008·Apr·9; 3(4):e1936. <·PMID:·18398462·>
• Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen‑presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008·May·15; 111(10):5037‑46. <·PMID:·18337560·>
• Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2‑related inducible co‑stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003·May; 126(5):1026‑35. <·PMID:·12690043·>
• Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL‑2, IL‑4, IL‑7 and IL‑15 preserve optimal suppressive potency in vitro. Int·Immunol. 2007·Jun; 19(6):785‑99. <·PMID:·17545278·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ IgG1 PerCP-Cy5.5 (MOPC-21) CZ886982 TDS (US)