フローサイトメーター

世界のリサーチ分野のお客さまへ先端の検査装置のご案内です。

  • 神戸:078-992-6272
  • 東京:03-5434-8556

【受付時間09:00~17:35※土日休日を除く/その他休日はサイト上で告知

お問い合わせ

sysmex

フローサイトメーターsysmex

CyFlow CD63 PerCP-Cy5.5

品番 CZ814010
抗体名 Anti-Hu CD63 PERCP-CY5.5,MEM-259
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PerCP-Cy5.5 (CZ886982)
反応性|交差吸着 Human レーザー Blue, Green
抗原 CD63, LIMP, LAMP-3, MLA1 最大蛍光波長 695 nm
クローン MEM-259 最大励起波長 482 nm
ホスト Mouse 標識/Format PerCP-Cy5.5
アイソタイプ IgG1 研究分野 Immunophenotyping | Endocytosis
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD63 PERCP-CY5.5,MEM-259

特異性

The mouse monoclonal antibody MEM-259 recognizes CD63 antigen, a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

抗原情報

CD63 (LAMP-3; lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T: The small chemical vacuolin‑1 inhibits Ca(2+)‑dependent lysosomal exocytosis but not cell resealing. EMBO·Rep. 2004·Sep; 5(9):883‑8. <·PMID:·15332114·>
• Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP‑1 and LAMP‑2, but not LAMP‑3, are reliable markers for activation‑induced secretion of human mast cells. Cytometry·A. 2004·Sep; 61(1):62‑8. <·PMID:·15351990·>
• Israels SJ, McMillan-Ward EM: CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb·Haemost. 2005·Feb; 93(2):311‑8. <·PMID:·15711748·>
• Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ: CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung. Lung·Cancer. 2007·Jul; 57(1):46‑53. <·PMID:·17350713·>
• Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH: Expression of tetraspan protein CD63 activates protein tyrosine kinase (PTK) and enhances the PTK‑induced inhibition of ROMK channels. J·Biol·Chem. 2008·Jan·22; <·PMID:·19880522·>
• Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to theendosomal‑lysosomal‑MIICs route after extracellular stimuli in human immature dendritic cells. Blood. 2004·Aug·15; 104(4):1183‑90. <·PMID:·15130945·>
• Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W: CD63 as an activation‑linked T cell costimulatory element. J·Immunol. 2004·Nov·15; 173(10):6000‑8. <·PMID:·15528334·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD63 PerCP-Cy5.5 (MEM-259) CZ814010 TDS (US)