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CyFlow CD28 Alexa Fluor 700

品番 CY207774
抗体名 Anti-Hu CD28 AF 700,CD28.2
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Alexa Fluor™ 700 (AS153909)
反応性|交差吸着 Human | Non-Human Primates レーザー Red
抗原 CD28, Tp44 最大蛍光波長 723 nm
クローン CD28.2 最大励起波長 702 nm
ホスト Mouse 標識/Format Alexa Fluor™ 700
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD28 AF 700,CD28.2

特異性

The mouse monoclonal antibody CD28.2 recognizes CD28 antigen, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

抗原情報

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7‑mediated stimulation of NK cells. J·Immunol. 1999·Jul·1; 163(1):62‑7. <·PMID:·10384130·>
• Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ: Immunization with hepatitis C virus‑like particles induces humoral and cellular immune responses in nonhuman primates. J·Virol. 2004·Jul; 78(13):6995‑7003. <·PMID:·15194776·>
• Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative‑feedback regulation of CD28 costimulation by a novel mitogen‑activated protein kinase phosphatase, MKP6. J·Immunol. 2001·Jan·1; 166(1):197‑206. <·PMID:·11123293·>
• Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T‑cell activation pathway involves an inosito phospholipid‑specific phospholipase C activity. Biochem·J. 1993·Aug·1; 293 (3):835‑42. <·PMID:·8394695·>
• Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int·Immunol. 1993·Mar; 5(3):311‑5. <·PMID:·8385476·>
• Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T‑cell activation negatively regulates NF‑kappa B signaling. Mol·Cell·Biol. 2004·May; 24(9):3860‑73. <·PMID:·15082780·>
• Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J·Immunol. 1999·Sep·15; 163(6):3511‑5. <·PMID:·10477625·>