フローサイトメーター

世界のリサーチ分野のお客さまへ先端の検査装置のご案内です。

  • 神戸:078-992-6272
  • 東京:03-5434-8556

【受付時間09:00~17:35※土日休日を除く/その他休日はサイト上で告知

お問い合わせ

sysmex

フローサイトメーターsysmex

CyFlow CD30 FITC

品番 CX511141
抗体名 Anti-Hu CD30 FITC,Ber-H8
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 FITC (CD051682)
反応性|交差吸着 Human レーザー Blue
抗原 CD30, D1S166E, Ki-1, TNFRSF8 最大蛍光波長 518 nm
クローン Ber-H8 最大励起波長 490 to 495 nm
ホスト Mouse 標識/Format FITC
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD30 FITC,Ber-H8

特異性

The mouse monoclonal antibody Ber-H8 recognizes extracellular part of CD30 antigen, a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).

抗原情報

CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Aalberse JA, Kapitein B, de Roock S, Klein MR, de Jager W, van der Zee R, Hoekstra MO, van Wijk F, Prakken BJ: Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). PLoS·One. 2011; 6(9):e24119. <·PMID:·21931651·>
• Berro AI, Perry GA, Agrawal DK: Increased expression and activation of CD30 induce apoptosis in human blood eosinophils. J·Immunol. 2004·Aug·1; 173(3):2174‑83. <·PMID:·15265955·>
• Falini B, Pileri S, Pizzolo G, Dürkop H, Flenghi L, Stirpe F, Martelli MF, Stein H: CD30 (Ki‑1) molecule: a new cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and immunotherapy. Blood. 1995·Jan·1; 85(1):41640. <·PMID:·7803786·>
• Franke AC, Jung D, Ellis TM: Characterization of the CD30L binding domain on the human CD30 molecule using anti‑CD30 antibodies. Hybridoma. 2000·Feb; 19(1):43‑8. <·PMID:·10768840·>
• Matsumoto K, Terakawa M, Miura K, Fukuda S, Nakajima T, Saito H: Extremely rapid and intense induction of apoptosis in human eosinophils by anti‑CD30 antibody treatment in vitro. J·Immunol. 2004·Feb·15; 172(4):2186‑93. <·PMID:·14764685·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD30 FITC (Ber-H8) CX511141 TDS (US)