特異性

The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

抗原情報

Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase‑3beta regulates cyclin D1 proteolysis and subcellular localization. Genes·Dev. 1998·Nov·15; 12(22):3499‑511. <·PMID:·9832503·>
• Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine‑286 prevents its rapid degradation via the ubiquitin‑proteasome pathway. Genes·Dev. 1997·Apr·15; 11(8):957‑72. <·PMID:·9136925·>
• Mukherji A, Janbandhu VC, Kumar V: GSK‑3beta‑dependent destabilization of cyclin D1 mediates replicational stress‑induced arrest of cell cycle. FEBS·Lett. 2008·Mar·6; <·PMID:·23416295·>
• Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3‑kinase/AKT/GSK‑3 beta pathway and is antagonized by insulin‑like growth factor 1. J·Biol·Chem. 2007·Feb·9; 282(6):3799‑808. <·PMID:·17130121·>