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CyFlow CD152 PE

品番 CV021049
抗体名 Anti-Hu CD152 PE,BNI3
包装単位 100 tests
濃度 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG2a PE (BY503796)
反応性|交差吸着 Human レーザー Blue, Green, Yellow
抗原 CD152, CTLA-4, CTL Ag 4 最大蛍光波長 576 nm
クローン BNI3 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG2a 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD152 PE,BNI3

特異性

The mouse monoclonal antibody BNI3 recognizes human CD152 antigen, a 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.

抗原情報

CD152 (CTLA-4) is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Chin LT, Chu C, Chen HM, Hsu SC, Weng BC, Chu CH: Site‑directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA‑4 (CD152) without interfering the engagement of natural ligands. BMC·Biotechnol. 2007·Aug·23; 7:51. <·PMID:·17714596·>
• Kraszula L, Eusebio M, Kupczyk M, Kuna P, Pietruczuk M: The use of multi‑color flow cytometry for identification of functional markers of nTregs in patients with severe asthma. Pneumonol·Alergol·Pol. 2012; 80(5):389‑401. <·PMID:·22926900·>
• Steiner K, Moosig F, Csernok E, Selleng K, Gross WL, Fleischer B, Bröker BM: Increased expression of CTLA‑4 (CD152) by T and B lymphocytes in Wegener's granulomatosis. Clin·Exp·Immunol. 2001·Oct; 126(1):143‑50. <·PMID:·11678911·>
• Steiner K1, Waase I, Rau T, Dietrich M, Fleischer B, Bröker BM: Enhanced expression of CTLA‑4 (CD152) on CD4+ T cells in HIV infection. Clin·Exp·Immunol. 1999·Mar; 115(3):451‑7. <·PMID:·10193417·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD152 PE (BNI3) CV021049 TDS (US)