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CyFlow CD33 PerCP-Cy5.5

品番 CU649508
抗体名 Anti-Hu CD33 PERCP-CY5.5,WM53
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PerCP-Cy5.5 (CZ886982)
反応性|交差吸着 Human | Non-Human Primates レーザー Blue, Green
抗原 CD33, p67, SIGLECT-3 最大蛍光波長 695 nm
クローン WM53 最大励起波長 482 nm
ホスト Mouse 標識/Format PerCP-Cy5.5
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD33 PERCP-CY5.5,WM53

特異性

The mouse monoclonal antibody WM53 recognize CD33 antigen, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

抗原情報

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bradstock KF, Kirk J, Grimsley PG, Kabral A, Hughes WG: Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br·J·Haematol. 1989·Aug; 72(4):512‑8. <·PMID:·2673329·>
• Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid‑differentiation antigen, 'gp67' (CD‑33). Dis·Markers. 1987·Dec; 5(4):215‑25. <·PMID:·3502990·>
• Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160, 95, gp150, gp67) investigation of epitopic heterogeneity and non‑haemopoietic distributionusing panels of monoclonal antibodies belonging to CD‑11b, CD‑13 and CD‑33. Br·J·Haematol. 1988·Jun; 69(2):163‑71. <·PMID:·2455535·>
• Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, de Tailly PD, Bulabois B, Poulet J, Kuhlein E, Jacob MC, Salaun V, Arock M, Drenou B, Schillinger F, Seilles E, Tiberghien P, Bensa JC, Plumas J, Saas P: Expression of the myeloid‑associated marker CD33 isnot an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005·Feb·1; 105(3):1256‑64. <·PMID:·15388576·>
• Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P: A study of CD33 (SIGLEC‑3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J·Leukoc·Biol. 2006·Jan; 79(1):46‑58. <·PMID:·16380601·>
• Hernández-López C, Varas A, Sacedón R, Jiménez E, Muñoz JJ, Zapata AG, Vicente A: Stromal cell‑derived factor 1/CXCR4 signaling is critical for early human T‑cell development. Blood. 2002·Jan·15; 99(2):546‑54. <·PMID:·11781237·>
• Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow‑derived stem cells after myocardial infarction and left ventricular function. Eur·Heart·J. 2005·Jun; 26(12):1196‑204. <·PMID:·15734770·>
• Schenk M, Bouchon A, Birrer S, Colonna M, Mueller C: Macrophages expressing triggering receptor expressed on myeloid cells‑1 are underrepresented in the human intestine. J·Immunol. 2005·Jan·1; 174(1):517‑24. <·PMID:·15611278·>
• Shin YK, Choi EY, Kim SH, Chung J, Chung DH, Park WS, Jung KC, Kim HS, Park S, Kim HJ, Park MH, Min CK, Kim CC, Park SH: Expression of leukemia‑associated antigen, JL1, in bone marrow and thymus. Am·J·Pathol. 2001·Apr; 158(4):1473‑80. <·PMID:·11290565·>
• Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC: Surface expression and function of p75/AIRM‑1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc·Natl·Acad·Sci·USA. 2001·May·8; 98(10):5764‑9. <·PMID:·11320212·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD33 PerCP-Cy5.5 (WM53) CU649508 TDS (US)