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CyFlow CD43 Low Endotoxin

品番 CU046978
抗体名 Anti-Hu CD43 LOW END,MEM-59
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Low Endotoxin (AK478344)
反応性|交差吸着 Human レーザー
抗原 CD43, Leukosialin, sialophorin 最大蛍光波長 -
クローン MEM-59 最大励起波長 -
ホスト Mouse 標識/Format Low Endotoxin
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Western blot
Immunohistochmistry (paraffin-embedded sections)
Functional assays

Anti-Hu CD43 LOW END,MEM-59

特異性

The mouse monoclonal antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 antigen, a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.

抗原情報

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM‑59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur·J·Immunol. 1995·Apr; 25(4):1051‑5. <·PMID:·7537669·>
• Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):21‑33. <·PMID:·17098248·>
• Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004·Apr·1; 23(14):2523‑30. <·PMID:·14676827·>
• Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T
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AbstractSend to:
Oncogene. 2008 Mar 13;27(12):1705-15. Epub 2007 Sep 24.
CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF.
Kadaja-Saarepuu L1, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T.: CD43 promotes cell growth and helps to evade FAS‑mediated apoptosis in non‑hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2008·Mar·13; 27(12):1705‑15. <·PMID:·17891181·>
• Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H: LFA‑1‑mediated leukocyte adhesion regulated by interaction of CD43 with LFA‑1 and CD147. Mol·Immunol. 2007·Nov·8; <·PMID:·19203795·>
• Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T‑cell trafficking. Blood. 2007·Oct·15; 110(8):2974‑82. <·PMID:·17638845·>
• Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL‑R1/DR4 to the plasma membrane. Apoptosis. 2008·Mar; 13(3):423‑36. <·PMID:·18165900·>
• Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia·Biol·(Praha). 1988; 34(4):255‑65. <·PMID:·2467832·>
• Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM‑43 anchored in membrane through phosphatidylinositol. Mol·Immunol. 1989·Feb; 26(2):153‑61. <·PMID:·2918859·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD43 Low Endotoxin (MEM-59) CU046978 TDS (US)