CyFlow IFN-gamma PerCP-Cy5.5
| 品番 | CS240755 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu IFN-GAM PERCPCY5.5,4S.B3 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PerCP-Cy5.5 (CZ886982) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | Blue, Green |
| 抗原 | IFN-γ | 最大蛍光波長 | 695 nm |
| クローン | 4S.B3 | 最大励起波長 | 482 nm |
| ホスト | Mouse | 標識/Format | PerCP-Cy5.5 |
| アイソタイプ | IgG1 | 研究分野 | Signaling |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu IFN-GAM PERCPCY5.5,4S.B3
特異性
The mouse monoclonal antibody 4S.B3 recognizes IFN-γ antigen, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
抗原情報
The Interferon γ (IFN-γ) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-α and IL-12; its production is suppressed by IL-4, IL-10, and TGF-β. The production of IFN-γ is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-γ polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-γ belongs to the type II interferons, also called immune IFN. IFN-γ shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-γ plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-γ to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-γ is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumors. The activation of macrophages by IFN-γ is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-γ can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-α, IFN-β). IFN-γ may also activate neutrophils and NK cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Brattig NW, Lepping B, Timmann C, Büttner DW, Marfo Y, Hamelmann C, Horstmann RD: Onchocerca volvulus‑exposed persons fail to produce interferon‑gamma in response to O. volvulus antigen but mount proliferative responses with interleukin‑5 and IL‑13 production that decrease with increasing microfilarial density. J·Infect·Dis. 2002·Apr·15; 185(8):1148‑54. <·PMID:·11930325·>
• Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM: Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte‑macrophage colony‑stimulating factor in the presence of glucocorticoids. Immunology. 1999·Sep; 98(1):104‑10. <·PMID:·10469240·>
• Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, Weetman A, Hale G, Chatterjee VK, Waldmann H, Compston A: Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999·Nov·13; 354(9191):1691‑5. <·PMID:·10568572·>
• Kosub DA, Lehrman G, Milush JM, Zhou D, Chacko E, Leone A, Gordon S, Silvestri G, Else JG, Keiser P, Jain MK, Sodora DL: Gamma/Delta T‑Cell Functional Responses Differ after Pathogenic Human Immunodeficiency Virus and Nonpathogenic Simian Immunodeficiency Virus Infections. J·Virol. 2008·Feb; 82(3):1155‑1165. <·PMID:·18045946·>
• Erik L Brincks, Tamara A Kucaba, Kevin L Legge, Thomas S Griffith: Influenza‑induced expression of functional TNF‑related apoptosis‑inducing ligand (TRAIL) on human PBMC. Hum·Immunol. 2008·Oct; 69(10):634‑646. <·PMID:·18723061·>
• Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA: Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006·Jul; 118(3):353‑60. <·PMID:·16827896·>
• Meager A, Parti S, Barwick S, Spragg J, O'Hagan K: Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. J·Interferon·Res. 1984·Fall; 4(4):619‑25. <·PMID:·6438252·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN