特異性

The mouse monoclonal antibody S5/1 recognizes the CD88 antigen, a 43 kDa receptor of C5a component of the complement cascade.

抗原情報

CD88 (C5aR) is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Cain SA, Monk PN: The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des‑Arg(74). J·Biol·Chem. 2002·Mar·1; 277(9):7165‑9. <·PMID:·11773063·>
• Camous L, Roumenina L, Bigot S, Brachemi S, Frémeaux-Bacchi V, Lesavre P, Halbwachs-Mecarelli L: Complement alternative pathway acts as a positive feedback amplification of neutrophil activation. Blood. 2011·Jan·27; 117(4):1340‑9. <·PMID:·21063021·>
• Conway Morris A, Kefala K, Wilkinson TS, Dhaliwal K, Farrell L, Walsh T, Mackenzie SJ, Reid H, Davidson DJ, Haslett C, Rossi AG, Sallenave JM, Simpson AJ: C5a mediates peripheral blood neutrophil dysfunction in critically ill patients. Am·J·Respir·Crit·Care·Med. 2009·Jul·1; 180(1):19‑28. <·PMID:·19324972·>
• Kiener HP, Baghestanian M, Dominkus M, Walchshofer S, Ghannadan M, Willheim M, Sillaber C, Graninger WB, Smolen JS, Valent P: Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis. Arthritis·Rheum. 1998·Feb; 41(2):233‑45. <·PMID:·9485081·>