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CyFlow CD4 PE

品番 CR188745
抗体名 Anti-Ms CD4 PE,GK1.5
包装単位 0.1 mg
濃度 0.5 mg/ml
容量 0.2 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 Mouse レーザー Blue, Green, Yellow
抗原 CD4, T4, Leu3a 最大蛍光波長 576 nm
クローン GK1.5 最大励起波長 496 nm, 565 nm
ホスト Rat 標識/Format PE
アイソタイプ IgG2b 研究分野 Immunophenotyping | MHC
クローナリティ monoclonal 用途

Flow cytometry

Anti-Ms CD4 PE,GK1.5

特異性

The rat monoclonal antibody GK1.5 recognizes an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).

抗原情報

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

利用方法

The reagent is designed for Flow Cytometry analysis. Suggested working usage is 3 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu‑3/T4 molecule. J·Immunol. 1983·Nov; 131(5):2445‑51. <·PMID:·6415170·>
• Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen‑reactivity. Immunol·Rev. 1983; 74:29‑56. <·PMID:·6195085·>
• Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide‑MHC complexes. Nat·Immunol. 2007·Apr; 8(4):388‑97. <·PMID:·17322886·>
• Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen‑induced airway hyperreactivity and pulmonary eosinophilia. Am·J·Respir·Cell·Mol·Biol. 1994·Jun; 10(6):587‑93. <·PMID:·8003337·>
• Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL‑12 influences intrathymic T cell development. J·Immunol. 1994·Mar·15; 152(6):2729‑35. <·PMID:·7511624·>
• Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long‑term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J·Immunol. 2008·May·15; 180(10):6593‑603. <·PMID:·18453578·>
• Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity: monoclonal antibody GK1.5 (anti‑L3T4a) blocks class II MHC antigen‑specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J·Immunol. 1983·Nov; 131(5):2178‑83. <·PMID:·6195255·>
• Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J·Exp·Med. 1991·Dec·1; 174(6):1617‑27. <·PMID:·1683894·>
• Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti‑CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008·Apr·27; 85(8):1167‑74. <·PMID:·18431238·>
• Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen‑specific emigrants that downregulate Thy‑1. J·Exp·Med. 1996·Sep·1; 184(3):1083‑91. <·PMID:·9064325·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD4 PE (GK1.5) CR188745 TDS (US)