特異性

The mouse monoclonal antibody HLy9.25 (HLy9.1.25) recognizes CD229 antigen, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

抗原情報

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bund D, Mayr C, Kofler DM, Hallek M, Wendtner CM: Human Ly9 (CD229 as novel tumor‑associated antigen (TAA) in chronic lymphocytic leukemia (B‑CLL) recognized by autologous CD8+ T cells. Exp·Hematol. 2006·Jul; 34(7):860‑9. <·PMID:·16797413·>
• de la Fuente MA, Tovar V, Villamor N, Zapater N, Pizcueta P, Campo E, Bosch J, Engel P: Molecular characterization and expression of a novel human leukocyte cell‑surface marker homologous to mouse Ly‑9. Blood. 2001·Jun·1; 97(11):3513‑20. <·PMID:·11369645·>
• Del Valle JM, Engel P, Martín M: The cell surface expression of SAP‑binding receptor CD229 is regulated via its interaction with clathrin‑associated adaptor complex 2 (AP‑2). J·Biol·Chem. 2003·May·9; 278(19):17430‑7. <·PMID:·12621057·>
• Martín M, Del Valle JM, Saborit I, Engel P: Identification of Grb2 as a novel binding partner of the signaling lymphocytic activation molecule‑associated protein binding receptor CD229. J·Immunol. 2005·May·15; 174(10):5977‑86. <·PMID:·15879090·>
• Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT‑2‑binding leukocyte cell‑surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue·Antigens. 2004·Aug; 64(2):132‑44. <·PMID:·15245368·>
• Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J·Immunol. 2005·Jun·1; 174(11):7033‑42. <·PMID:·15905546·>
• Sintes J, Romero X, Marin P, Terhorst C, Engel P: Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells. Exp·Hematol. 2008·Sep; 36(9):1199‑204. <·PMID:·18495325·>