CyFlow HLA-Class I Purified
品番 | CQ351748 | ||
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抗体名 | Anti-Hu HLA-ClAss I PURI,MEM-147 | ||
包装単位 | 0.1 mg | ||
濃度 | 1 mg/ml | ||
容量 | 0.1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 Purified (BJ561896) Mouse IgG1 Purified (AS224818) |
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反応性|交差吸着 | Human | レーザー | |
抗原 | HLA-Class I | 最大蛍光波長 | - |
クローン | MEM-147 | 最大励起波長 | - |
ホスト | Mouse | 標識/Format | Purified |
アイソタイプ | IgG1 | 研究分野 | MHC |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu HLA-ClAss I PURI,MEM-147
特異性
The mouse monoclonal antibody MEM-147 recognizes all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
抗原情報
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant β2-microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
利用方法
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·>
• Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid‑rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998·May·15; 91(10):3901‑8. <·PMID:·9573028·>
• Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan‑HLA class I‑reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA‑B27/beta2‑microglobulin complex. Immunogenetics. 2001·Aug; 53(6):440‑6. <·PMID:·11685454·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN