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CyFlow CD46 PE

品番 CP264261
抗体名 Anti-Hu CD46 PE,MEM-258
包装単位 100 tests
濃度 -
容量 2 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human | Cow レーザー Blue, Green, Yellow
抗原 CD46, MCP 最大蛍光波長 576 nm
クローン MEM-258 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD46 PE,MEM-258

特異性

The mouse monoclonal antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 antigen, a 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.

抗原情報

CD46 (MCP; membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Fleischli C, Sirena D, Lesage G, Havenga MJ, Cattaneo R, Greber UF, Hemmi S: Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor. J·Gen·Virol. 2007·Nov; 88(11):2925‑34. <·PMID:·17947513·>
• Fleischli C, Verhaagh S, Havenga M, Sirena D, Schaffner W, Cattaneo R, Greber UF, Hemmi S: The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35. J·Virol. 2005·Aug; 79(15):10013‑22. <·PMID:·16014961·>
• Fremeaux-Bacchi V, Moulton EA, Kavanagh D, Dragon-Durey MA, Blouin J, Caudy A, Arzouk N, Cleper R, Francois M, Guest G, Pourrat J, Seligman R, Fridman WH, Loirat C, Atkinson JP: Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome. J·Am·Soc·Nephrol. 2006·Jul; 17(7):2017‑25. <·PMID:·16762990·>
• Gaggar A, Shayakhmetov DM, Lieber A: CD46 is a cellular receptor for group B adenoviruses. Nat·Med. 2003·Nov; 9(11):1408‑12. <·PMID:·14566335·>
• Hoffmann D, Bayer W, Heim A, Potthoff A, Nettelbeck DM, Wildner O: Evaluation of twenty‑one human adenovirus types and one infectivity‑enhanced adenovirus for the treatment of malignant melanoma. J·Invest·Dermatol. 2008·Apr; 128(4):988‑98. <·PMID:·17960177·>
• Kälin S, Amstutz B, Gastaldelli M, Wolfrum N, Boucke K, Havenga M, DiGennaro F, Liska N, Hemmi S, Greber UF: Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35. J·Virol. 2010·May; 84(10):5336‑50. <·PMID:·20237079·>
• Liszewski MK, Kemper C, Price JD, Atkinson JP: Emerging roles and new functions of CD46. Springer·Semin·Immunopathol. 2005·Nov; 27(3):345‑58. <·PMID:·16200405·>
• Loré K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J, Koup RA: Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J·Immunol. 2007·Aug·1; 179(3):1721‑9. <·PMID:·17641038·>
• Marie JC, Astier AL, Rivailler P, Rabourdin-Combe C, Wild TF, Horvat B: Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation. Nat·Immunol. 2002·Jul; 3(7):659‑66. <·PMID:·12055630·>
• Okada N, Liszewski MK, Atkinson JP, Caparon M: Membrane cofactor protein (CD46 is a keratinocyte receptor for the M protein of the group A streptococcus. Proc·Natl·Acad·Sci·USA. 1995·Mar·28; 92(7):2489‑93. <·PMID:·7708671·>
• Oliaro J, Pasam A, Waterhouse NJ, Browne KA, Ludford-Menting MJ, Trapani JA, Russell SM: Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation. Proc·Natl·Acad·Sci·USA. 2006·Dec·5; 103(49):18685‑90. <·PMID:·17116876·>
• Rebetz J, Na M, Su C, Holmqvist B, Edqvist A, Nyberg C, Widegren B, Salford LG, Sjögren HO, Arnberg N, Qian Q, Fan X: Fiber mediated receptor masking in non‑infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co‑existence. PLoS·One. 2009·Dec·29; 4(12):e8484. <·PMID:·20041185·>
• Wang H, Tuve S, Erdman DD, Lieber A: Receptor usage of a newly emergent adenovirus type 14. Virology. 2009·May·10; 387(2):436‑41. <·PMID:·19307010·>
• Weyand NJ, Lee SW, Higashi DL, Cawley D, Yoshihara P, So M: Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies. Infect·Immun. 2006·Apr; 74(4):2428‑35. <·PMID:·16552073·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD46 PE (MEM-258) CP264261 TDS (US)