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CyFlow CD4 Azide Free

品番 CN672458
抗体名 Anti-Ms CD4 AZIDFREE,GK1.5
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 Mouse レーザー
抗原 CD4, T4, Leu3a 最大蛍光波長 -
クローン GK1.5 最大励起波長 -
ホスト Rat 標識/Format Azide Free
アイソタイプ IgG2b 研究分野 Immunophenotyping | MHC
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Immunohistochemistry (frozen sections)
Immunocytochemistry
Functional assays

Anti-Ms CD4 AZIDFREE,GK1.5

特異性

The rat monoclonal antibody GK1.5 recognizes an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).

抗原情報

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu‑3/T4 molecule. J·Immunol. 1983·Nov; 131(5):2445‑51. <·PMID:·6415170·>
• Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen‑reactivity. Immunol·Rev. 1983; 74:29‑56. <·PMID:·6195085·>
• Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide‑MHC complexes. Nat·Immunol. 2007·Apr; 8(4):388‑97. <·PMID:·17322886·>
• Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen‑induced airway hyperreactivity and pulmonary eosinophilia. Am·J·Respir·Cell·Mol·Biol. 1994·Jun; 10(6):587‑93. <·PMID:·8003337·>
• Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL‑12 influences intrathymic T cell development. J·Immunol. 1994·Mar·15; 152(6):2729‑35. <·PMID:·7511624·>
• Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long‑term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J·Immunol. 2008·May·15; 180(10):6593‑603. <·PMID:·18453578·>
• Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity: monoclonal antibody GK1.5 (anti‑L3T4a) blocks class II MHC antigen‑specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J·Immunol. 1983·Nov; 131(5):2178‑83. <·PMID:·6195255·>
• Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J·Exp·Med. 1991·Dec·1; 174(6):1617‑27. <·PMID:·1683894·>
• Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti‑CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008·Apr·27; 85(8):1167‑74. <·PMID:·18431238·>
• Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen‑specific emigrants that downregulate Thy‑1. J·Exp·Med. 1996·Sep·1; 184(3):1083‑91. <·PMID:·9064325·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD4 Azide Free (GK1.5) CN672458 TDS (US)