特異性

The Armenian hamster monoclonal antibody 16-10A1 recognizes CD80 antigen, a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

抗原情報

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Gueirard P, Ave P, Balazuc AM, Thiberge S, Huerre M, Milon G, Guiso N: Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect·Immun. 2003·Jul; 71(7):4137‑43. <·PMID:·12819105·>
• Horspool JH, Perrin PJ, Woodcock JB, Cox JH, King CL, June CH, Harlan DM, St Louis DC, Lee KP: Nucleic acid vaccine‑induced immune responses require CD28 costimulation and are regulated by CTLA4. J·Immunol. 1998·Mar·15; 160(6):2706‑14. <·PMID:·9510170·>
• Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L: CD80+Gr‑1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis. J·Immunol. 2002·Sep·15; 169(6):3180‑90. <·PMID:·12218136·>
• Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, Holdsworth SR: Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis. J·Am·Soc·Nephrol. 2005·Jul; 16(7):2012‑22. <·PMID:·15944341·>
• Paust S, Lu L, McCarty N, Cantor H: Engagement of B7 on effector T cells by regulatory T cells prevents autoimmune disease. Proc·Natl·Acad·Sci·USA. 2004·Jul·13; 101(28):10398‑403. <·PMID:·15235129·>
• Quah B, Ni K, O'Neill HC: In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity. Int·Immunol. 2004·Apr; 16(4):567‑77. <·PMID:·15039387·>
• Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P: Induction of B7‑1 in podocytes is associated with nephrotic syndrome. J·Clin·Invest. 2004·May; 113(10):1390‑7. <·PMID:·15146236·>
• Mukherjee S, Ahmed A, Malu S, Nandi D: Modulation of cell cycle progression by CTLA4‑CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL‑2. J·Leukoc·Biol. 2006·Jul; 80(1):66‑74. <·PMID:·16624934·>