CyFlow CD59 Purified
品番 | CM335631 | ||
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抗体名 | Anti-H/M CD59 PURI,MEM-43/5 | ||
包装単位 | 0.1 mg | ||
濃度 | 1 mg/ml | ||
容量 | 0.1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG2b Purified (AS131472) Mouse IgG2b Purified (CK823368) |
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反応性|交差吸着 | Human | Mouse | レーザー | |
抗原 | CD59, Protectin H19 | 最大蛍光波長 | - |
クローン | MEM-43/5 | 最大励起波長 | - |
ホスト | Mouse | 標識/Format | Purified |
アイソタイプ | IgG2b | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-H/M CD59 PURI,MEM-43/5
特異性
The mouse monoclonal antibody MEM-43/5 recognizes well defined epitope (around L33) on CD59 antigen, a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.
抗原情報
CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
利用方法
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Awasthi S, Wolf R, White G: Ontogeny and phagocytic function of baboon lung dendritic cells. Immunol·Cell·Biol. 2009·Jul; 87(5):419‑27. <·PMID:·19223853·>
• Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement‑inhibitory glycoprotein, CD59. J·Exp·Med. 1997·Feb·3; 185(3):507‑16. <·PMID:·9053451·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·>
• Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·>
• Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J·Immunol. 2007·Feb·15; 178(4):2262‑71. <·PMID:·17277131·>
• Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J·Immunol. 2005·Jun·1; 174(11):7033‑42. <·PMID:·15905546·>
• Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly‑6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J·Transl·Med. 2004·Mar·29; 2(1):8. <·PMID:·15050027·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN