CyFlow CD3 act. PE
品番 | CM152332 | ||
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抗体名 | Anti-Hu/Ms CD3 ACT. PE,APA1/1 | ||
包装単位 | 0.1 mg | ||
濃度 | 0.1 mg/ml | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
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反応性|交差吸着 | Human | Mouse | レーザー | Blue, Green, Yellow |
抗原 | CD3 (activation epitope) | 最大蛍光波長 | 576 nm |
クローン | APA1/1 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu/Ms CD3 ACT. PE,APA1/1
特異性
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 ε. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation
抗原情報
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 γ, CD3 δ, CD3 ε and CD3 ζ. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Alarcón B, Ley SC, Sánchez-Madrid F, Blumberg RS, Ju ST, Fresno M, Terhorst C: The CD3‑gamma and CD3‑delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes. EMBO·J. 1991·Apr; 10(4):903‑12. <·PMID:·1826255·>
• Delgado P, Fernández E, Dave V, Kappes D, Alarcón B: CD3delta couples T‑cell receptor signalling to ERK activation and thymocyte positive selection. Nature. 2000·Jul·27; 406(6794):426‑30. <·PMID:·10935641·>
• Gil D, Schrum AG, Alarcón B, Palmer E: T cell receptor engagement by peptide‑MHC ligands induces a conformational change in the CD3 complex of thymocytes. J·Exp·Med. 2005·Feb·21; 201(4):517‑22. <·PMID:·15728235·>
• Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·>
• Risueño RM, Gil D, Fernández E, Sánchez-Madrid F, Alarcón B: Ligand‑induced conformational change in the T‑cell receptor associated with productive immune synapses. Blood. 2005·Jul·15; 106(2):601‑8. <·PMID:·15790785·>
• Risueño RM, van Santen HM, Alarcón B: A conformational change senses the strength of T cell receptor‑ligand interaction during thymic selection. Proc·Natl·Acad·Sci·USA. 2006·Jun·20; 103(25):9625‑30. <·PMID:·16766661·>
• Tailor P, Tsai S, Shameli A, Serra P, Wang J, Robbins S, Nagata M, Szymczak-Workman AL, Vignali DA, Santamaria P: The proline‑rich sequence of CD3epsilon as an amplifier of low‑avidity TCR signaling. J·Immunol. 2008·Jul·1; 181(1):243‑55. <·PMID:·18566390·>
• Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·>
• Wong AP, Dutly AE, Sacher A, Lee H, Hwang DM, Liu M, Keshavjee S, Hu J, Waddell TK: Targeted cell replacement with bone marrow cells for airway epithelial regeneration. Am·J·Physiol·Lung·Cell·Mol·Physiol. 2007·Sep; 293(3):L740‑52. <·PMID:·17616650·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN