CyFlow HLA-DR1 (empty) PE
| 品番 | CM093224 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu HLA-DR1(EM) PE,MEM-267 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.1 mg/ml | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2b PE (CP922237) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | HLA-DR1 (empty) | 最大蛍光波長 | 576 nm |
| クローン | MEM-267 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG2b | 研究分野 | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu HLA-DR1(EM) PE,MEM-267
特異性
The mouse monoclonal antibody MEM-267 specifically binds to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
抗原情報
HLA-DR1 belongs to the HLA class II β chain paralogues. The MHC Class II molecule is a heterodimer consisting of an α (DRA) and a β chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The β chain is approximately 26-28 kDa. Within the DR molecule the β chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Carven GJ, Chitta S, Hilgert I, Rushe MM, Baggio RF, Palmer M, Arenas JE, Strominger JL, Horejsi V, Santambrogio L, Stern LJ: Monoclonal antibodies specific for the empty conformation of HLA‑DR1 reveal aspects of the conformational change associated with peptide binding. J·Biol·Chem. 2004·Apr·16; 279(16):16561‑70. <·PMID:·14757758·>
• Potolicchio I, Chitta S, Xu X, Fonseca D, Crisi G, Horejsi V, Strominger JL, Stern LJ, Raposo G, Santambrogio L: Conformational variation of surface class II MHC proteins during myeloid dendritic cell differentiation accompanies structural changes in lysosomal MIIC. J·Immunol. 2005·Oct·15; 175(8):4935‑47. <·PMID:·16210595·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN