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CyFlow CD4 Alexa Fluor 700

品番 CM002106
抗体名 Anti-Hu CD4 AF 700,MEM-241
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Alexa Fluor™ 700 (AS153909)
反応性|交差吸着 Human レーザー Red
抗原 CD4, T4, Leu3a 最大蛍光波長 723 nm
クローン MEM-241 最大励起波長 702 nm
ホスト Mouse 標識/Format Alexa Fluor™ 700
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD4 AF 700,MEM-241

特異性

The mouse monoclonal antibody MEM-241 recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes.

抗原情報

CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.

Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin
.
Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction.

Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Anderson AE, Sayers BL, Haniffa MA, Swan DJ, Diboll J, Wang XN, Isaacs JD, Hilkens CM: Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells. J·Leukoc·Biol. 2008·Jul; 84(1):124‑33. <·PMID:·18430785·>
• Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J·Exp·Med. 2003·Nov·17; 198(10):1453‑62. <·PMID:·14610046·>
• Clapham PR, McKnight A: Cell surface receptors, virus entry and tropism of primate lentiviruses. . J·Gen·Virol. 2002·Aug; 83(8):1809‑29. <·PMID:·12124446·>
• Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc·Natl·Acad·Sci·USA. 2002·Feb·19; 99(4):2008‑13. <·PMID:·11854499·>
• Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC·Immunol. 2011·Jan·5; 12:2. <·PMID:·21208424·>
• Karlsson KR, Cowley S, Martinez FO, Shaw M, Minger SL, James W: Homogeneous monocytes and macrophages from human embryonic stem cells following coculture‑free differentiation in M‑CSF and IL‑3. Exp·Hematol. 2008·Sep; 36(9):1167‑75. <·PMID:·18550257·>
• Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus‑infected patients in Zimbabwe. Clin·Vaccine·Immunol. 2007·Mar; 14(3):293‑8. <·PMID:·17267593·>
• Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent‑resistant T‑cell membrane microdomains. Tissue·Antigens. 1999·Jan; 53(1):33‑40. <·PMID:·10082429·>
• Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X: CD molecules 2006: human cell differentiation molecules. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):1‑5. <·PMID:·17174972·>