CyFlow BrdU PE
| 品番 | CL205376 | ||
|---|---|---|---|
| 抗体名 | BrdU PE,Bu20a | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | レーザー | Blue, Green, Yellow | |
| 抗原 | 5-bromodeoxyuridine | 最大蛍光波長 | 576 nm |
| クローン | Bu20a | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Small organic molecules |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
BrdU PE,Bu20a
特異性
The mouse monoclonal antibody Bu20a reacts specifically with 5-bromodeoxyuridine (BrdU) incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or IHC staining.
抗原情報
5-bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Lagares A, Li HY, Zhou XF, Avendaňo C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio‑neuronal precursor maturation. J·Neurosci. 2007·Jul·25; 27(30):7939‑53. <·PMID:·17652585·>
• Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti‑bromodeoxyuridine. J·Histochem·Cytochem. 1989·Oct; 37(10):1517‑27. <·PMID:·2476478·>
• Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005·Apr; 29(1):33‑8. <·PMID:·15954465·>
• Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki‑67(MIB‑5) IHC and in situ hybridization for histone mRNA. J·Histochem·Cytochem. 2003·Dec; 51(12):1681‑8. <·PMID:·14623936·>
• O'Driscoll M, Jeggo PA: CsA can induce DNA double‑strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair. Bone·Marrow·Transplant. 2008·Jun; 41(11):983‑9. <·PMID:·18278071·>
• Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J·Cell·Biol. 2007·Mar·12; 176(6):807‑18. <·PMID:·17353358·>
• Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'‑bromo‑2'‑deoxyuridine. Am·J·Physiol·Cell·Physiol. 2005·Sep; 289(3):C757‑65. <·PMID:·15857903·>
• Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques. Am·J·Physiol·Heart·Circ·Physiol. 2008·Feb; 294(2):H944‑53. <·PMID:·18083905·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN