CyFlow IgG2b Purified
| 品番 | CK823368 | ||
|---|---|---|---|
| 抗体名 | Mouse IGG2B PURI,PLRV219 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | レーザー | ||
| 抗原 | IgG2b Isotype Control | 最大蛍光波長 | - |
| クローン | PLRV219 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Purified |
| アイソタイプ | IgG2b | 研究分野 | Isotype controls |
| クローナリティ | monoclonal | 用途 | Flow cytometry (Isotype control) |
Mouse IGG2B PURI,PLRV219
特異性
This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.
抗原情報
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PLRV219 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
利用方法
The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10 µg/ml of incubation mixture), change the experimental conditions to reduce the background.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Glier H, Holada K: Blood storage affects the detection of cellular prion protein on peripheral blood leukocytes and circulating dendritic cells in part by promoting platelet satellitism.. J·Immunol·Methods. 2012·Jun·29; 380(1-2):65-72. <·PMID:·22522115·>
• Rygh CB, Wang J, Thuen M, Gras Navarro A, Huuse EM, Thorsen F, Poli A, Zimmer J, Haraldseth O, Lie SA, Enger PØ, Chekenya M: Dynamic Contrast Enhanced MRI Detects Early Response to Adoptive NK Cellular Immunotherapy Targeting the NG2 Proteoglycan in a Rat Model of Glioblastoma. PLoS·One. 2014·Sep·30; 9(9):e108414. <·PMID:·25268630·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN