CyFlow CD11a FITC
| 品番 | CK020205 | ||
|---|---|---|---|
| 抗体名 | Anti-Ms CD11A FITC,M17/4 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Mouse | レーザー | Blue |
| 抗原 | CD11a, LFA-1 | 最大蛍光波長 | 518 nm |
| クローン | M17/4 | 最大励起波長 | 490 to 495 nm |
| ホスト | Rat | 標識/Format | FITC |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms CD11A FITC,M17/4
特異性
The rat monoclonal antibody M17/4 recognizes mouse CD11a (α-subunit of murine LFA-1) antigen, a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
抗原情報
CD11a (LFA-1 α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 3 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC‑peptide complexes containing long rigid linkers abolish CTL‑mediated cytotoxicity. J·Immunol. 2006·Mar·15; 176(6):3356‑65. <·PMID:·16517703·>
• Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule‑dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J·Exp·Med. 2002·Dec·2; 196(11):1515‑21. <·PMID:·12461086·>
• Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir·Res. 2005·Jul·12; 6:70. <·PMID:·16011799·>
• Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int·Immunol. 2001·Sep; 13(9):1165‑74. <·PMID:·11526097·>
• Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule‑1 in T lymphocyte activation: Contributions of adhesion and co‑activation. J·Immunol. 1991·Mar·15; 146(6):1773‑82. <·PMID:·1672331·>
• Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2‑deficient mice. Blood. 2001·Mar·1; 97(5):1282‑8. <·PMID:·11222371·>
• Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T‑lymphocyte (CTL)‑mediated killing: functional screening and topographic relationship. Cell·Immunol. 1982·Oct; 73(1):1-11. <·PMID:·6983917·>
• Smith SS, Barnum SR: Differential expression of beta 2‑integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J·Leukoc·Biol. 2008·Jan; 83(1):71‑9. <·PMID:·17928460·>
• Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug‑induced lupus (II) T cells overexpressing lymphocyte function‑associated antigen 1 become autoreactive and cause a lupus‑like disease in syngeneic mice. J·Clin·Invest. 1996·Jun·15; 97(12):2866‑71. <·PMID:·8675699·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN