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CyFlow IgG2a Purified

品番 CJ747338
抗体名 Mouse IGG2A PURI,MOPC-173
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 レーザー
抗原 IgG2a Isotype Control 最大蛍光波長 -
クローン MOPC-173 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG2a 研究分野 Isotype controls
クローナリティ monoclonal 用途

Flow cytometry (Isotype control)

Mouse IGG2A PURI,MOPC-173

特異性

This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

抗原情報

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

利用方法

The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10 µg/ml of incubation mixture), change the experimental conditions to reduce the background.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J·Immunol. 1976·Jan; 116(1):65‑74. <·PMID:·812916·>
• Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann·Immunol·(Paris). 1976·Sep-Oct; 127(5):607‑31. <·PMID:·984731·>
• Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta‑tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007·Apr·15; 109(8):3513‑20. <·PMID:·17170130·>
• Khoddami V, Cairns BR: Transcriptome‑wide target profiling of RNA cytosine methyltransferases using the mechanism‑based enrichment procedure Aza‑IP. Nat·Protoc. 2014·Feb; 9(2):337‑61. <·PMID:·24434802·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ IgG2a Purified (MOPC-173) CJ747338 TDS (US)