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CyFlow CD38 APC

品番 CH560586
抗体名 Anti-Hu CD38 APC,HIT2
包装単位 100 tests
濃度 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 APC (AB294043)
反応性|交差吸着 Human レーザー Red
抗原 CD38, T10 最大蛍光波長 660 nm
クローン HIT2 最大励起波長 650 nm
ホスト Mouse 標識/Format APC
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD38 APC,HIT2

特異性

The mouse monoclonal antibody HIT2 recognizes CD38 antigen, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells.

抗原情報

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP‑ribose‑CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase‑catalysed reactions: implication for CD38 signalling. Biochem·J. 2001·Sep·1; 358(2):399‑406. <·PMID:·11513738·>
• Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007·Mar·15; 109(6):2545‑52. <·PMID:·17132718·>
• Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro‑B leukemic cell line by a tyrosine kinase‑dependent but ADP‑ribosyl cyclase‑ and NAD glycohydrolase‑independent mechanism. Int·Immunol. 2006·Jul; 18(7):1029‑42. <·PMID:·16720618·>
• Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE: Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp‑ribose, the major product generated by the CD38 enzymereaction. J·Immunol. 2007·Dec·1; 179(11):7827‑39. <·PMID:·18025229·>
• Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll‑like receptors on B‑CLL cells: expression and functional consequences of their stimulation. Int·J·Cancer. 2010·Mar·1; 126(5):1132‑43. <·PMID:·19685493·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD38 APC (HIT2) CH560586 TDS (US)