特異性

The rat monoclonal antibody M5/114 recognizes mouse MHC class II glycoproteins (MHCII). It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

抗原情報

MHC (major histocompatibility complex) class II (MHCII) molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding groove between MHC class II α and β chain is open at both ends and is 15-24 amino acid residues long.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II‑mediated antigen presentation by epithelial cells in vivo. J·Immunol. 2005·Feb·1; 174(3):1205‑12. <·PMID:·15661874·>
• Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I‑A and I‑E subregions: evidence for I region gene duplication. J·Immunol. 1981·Dec; 127(6):2488‑95. <·PMID:·6170707·>
• Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5‑deficient mice. Immunity. 1998·Feb; 8(2):143‑55. <·PMID:·9491996·>
• De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J·Cell·Sci. 1995·Oct; 108 (10):3219‑31. <·PMID:·7593283·>
• Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J·Cell·Biol. 2001·Oct·1; 155(1):53‑63. <·PMID:·11581285·>
• Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell‑surface MHC class II expression on B cells and other antigen‑presenting cells. Int·Immunol. 2007·Aug; 19(8):977‑92. <·PMID:·17804692·>
• Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II‑associated invariant chain. Cell. 1993·Feb·26; 72(4):635‑48. <·PMID:·7679955·>
• Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II‑mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J·Am·Soc·Nephrol. 2005·Dec; 16(12):3661‑8. <·PMID:·16221866·>