CyFlow CD19 PE
| 品番 | CF747469 | ||
|---|---|---|---|
| 抗体名 | Anti-Ms CD19 PE,1D3 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Mouse | レーザー | Blue, Green, Yellow |
| 抗原 | CD19, B4 | 最大蛍光波長 | 576 nm |
| クローン | 1D3 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Rat | 標識/Format | PE |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms CD19 PE,1D3
特異性
The rat monoclonal antibody 1D3 recognizes mouse CD19 antigen, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
抗原情報
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 1 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement‑tagged antigens. J·Immunol. 2001·Jul·1; 167(1):163‑72. <·PMID:·11418645·>
• Inabe K, Kurosaki T: Tyrosine phosphorylation of B‑cell adaptor for phosphoinositide 3‑kinase is required for Akt activation in response to CD19 engagement. Blood. 2002·Jan·15; 99(2):584‑9. <·PMID:·11781242·>
• Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self‑renewal of B‑1 lymphocytes is dependent on CD19. Eur·J·Immunol. 1996·Jan; 26(1):238‑42. <·PMID:·8566073·>
• Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J·Immunol. 1996·Jul·1; 157(1):48‑56. <·PMID:·8683154·>
• Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J·Immunol. 2003·Oct·15; 171(8):4062‑72. <·PMID:·14530327·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN