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CyFlow CD3 act. Purified

品番 CF594139
抗体名 Anti-Hu/Ms CD3 ACT. PURI,APA1/1
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Purified (BJ561896)
Mouse IgG1 Purified (AS224818)
反応性|交差吸着 Human | Mouse レーザー
抗原 CD3 (activation epitope) 最大蛍光波長 -
クローン APA1/1 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Western blot
Immunohistochemistry (frozen sections)
Immunocytochemistry

Anti-Hu/Ms CD3 ACT. PURI,APA1/1

特異性

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 ε. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation

抗原情報

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 γ, CD3 δ, CD3 ε and CD3 ζ. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Alarcón B, Ley SC, Sánchez-Madrid F, Blumberg RS, Ju ST, Fresno M, Terhorst C: The CD3‑gamma and CD3‑delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes. EMBO·J. 1991·Apr; 10(4):903‑12. <·PMID:·1826255·>
• Delgado P, Fernández E, Dave V, Kappes D, Alarcón B: CD3delta couples T‑cell receptor signalling to ERK activation and thymocyte positive selection. Nature. 2000·Jul·27; 406(6794):426‑30. <·PMID:·10935641·>
• Gil D, Schrum AG, Alarcón B, Palmer E: T cell receptor engagement by peptide‑MHC ligands induces a conformational change in the CD3 complex of thymocytes. J·Exp·Med. 2005·Feb·21; 201(4):517‑22. <·PMID:·15728235·>
• Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·>
• Risueño RM, Gil D, Fernández E, Sánchez-Madrid F, Alarcón B: Ligand‑induced conformational change in the T‑cell receptor associated with productive immune synapses. Blood. 2005·Jul·15; 106(2):601‑8. <·PMID:·15790785·>
• Risueño RM, van Santen HM, Alarcón B: A conformational change senses the strength of T cell receptor‑ligand interaction during thymic selection. Proc·Natl·Acad·Sci·USA. 2006·Jun·20; 103(25):9625‑30. <·PMID:·16766661·>
• Tailor P, Tsai S, Shameli A, Serra P, Wang J, Robbins S, Nagata M, Szymczak-Workman AL, Vignali DA, Santamaria P: The proline‑rich sequence of CD3epsilon as an amplifier of low‑avidity TCR signaling. J·Immunol. 2008·Jul·1; 181(1):243‑55. <·PMID:·18566390·>
• Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·>
• Wong AP, Dutly AE, Sacher A, Lee H, Hwang DM, Liu M, Keshavjee S, Hu J, Waddell TK: Targeted cell replacement with bone marrow cells for airway epithelial regeneration. Am·J·Physiol·Lung·Cell·Mol·Physiol. 2007·Sep; 293(3):L740‑52. <·PMID:·17616650·>